LOCUS       GALC-pGEn1-DEST         6508 bp    DNA     circular     25-JAN-2013
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    2977..3054
                     /note="TCM Sig Seq"
     misc_recomb     complement(3114..3142)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    3055..3078
                     /note="8xHis Tag"
     misc_feature    3085..3108
                     /note="StrepTag"
     misc_feature    5136..5677
                     /note="WPRE"
     misc_recomb     5098..5120
                     /label=attB2
                     /note="attB2 site"
     misc_feature    complement(5684..5898)
                     /note="BGH pA"
     misc_feature    complement(5986..6115)
                     /note="3'-ITR"
     misc_feature    3143..3165
                     /note="TEV site"
     source          3166..5097
                     /dnas_title="GALC-pGEn1-DEST"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:33117 IMAGE:5267705"
                     /tissue_type="Testis"
                     /clone_lib="NIH_MGC_97"
                     /lab_host="DH10B"
                     /note="Vector: pBluescriptR"
     gene            3166..5097
                     /dnas_title="GALC"
                     /gene="GALC"
                     /db_xref="GeneID:2581"
                     /db_xref="HGNC:4115"
                     /db_xref="MIM:606890"
     CDS             3166..5097
                     /dnas_title="GALC protein"
                     /gene="GALC"
                     /codon_start=1
                     /product="GALC protein"
                     /protein_id="AAH36518.1"
                     /db_xref="GI:22328079"
                     /db_xref="GeneID:2581"
                     /db_xref="HGNC:4115"
                     /db_xref="MIM:606890"
                     /translation="MTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGA
                     VSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMH
                     YALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVV
                     TWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESIS
                     ASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRIL
                     NQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQF
                     TQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQF
                     ATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTL
                     TTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPG
                     EHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTTKCDVYIETPDTGGVFIAGRV
                     NKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHF
                     ASGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR"
     misc_difference 3369..3369
                     /dnas_title="GALC"
                     /gene="GALC"
                     /note="'T' in cDNA is 'C' in the human genome; no amino
                     acid change.  The chimpanzee genome agrees with the human
                     genomic sequence and not the cDNA."
     misc_feature    3166..5097
                     /note="GALC coding region"
     misc_difference 4659..4659
                     /dnas_title="GALC"
                     /gene="GALC"
                     /note="'G' in cDNA is 'A' in the human genome; no amino
                     acid change."
     misc_difference 4724..4724
                     /dnas_title="GALC"
                     /gene="GALC"
                     /note="'C' in cDNA is 'T' in the human genome; amino acid
                     difference: 'T' in cDNA, 'I' in the human genome.  The
                     chimpanzee genome agrees with the human genomic sequence
                     and not the cDNA."
     misc_difference 4737..4737
                     /dnas_title="GALC"
                     /gene="GALC"
                     /note="'T' in cDNA is 'A' in the human genome; no amino
                     acid change."
     misc_difference 4960..4960
                     /dnas_title="GALC"
                     /gene="GALC"
                     /note="'G' in cDNA is 'A' in the human genome; amino acid
                     difference: 'A' in cDNA, 'T' in the human genome."
     vector          join(5106..6508,1..3132)
                     /source="pGEn1-DEST"
                     /type="Destination Vector"
     clone           3133..5105
                     /source="GALC-Nterm-TEVfusion-pDONR221"
                     /type="Entry Clone"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC
     3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC
     3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA
     3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggctacgt gctcgacgac
     3181 tccgacgggc tgggccggga gttcgacggc atcggcgcgg tcagcggcgg cggggcaacc
     3241 tcccgacttc tagtaaatta cccagagccc tatcgttctc agatattgga ttatctcttt
     3301 aagccgaatt ttggtgcctc tttgcatatt ttaaaagtgg aaataggtgg tgatgggcag
     3361 acaacagatg gcactgagcc ctcccacatg cattatgcac tagatgagaa ttatttccga
     3421 ggatacgagt ggtggttgat gaaagaagct aagaagagga atcccaatat tacactcatt
     3481 gggttgccat ggtcattccc tggatggctg ggaaaaggtt tcgactggcc ttatgtcaat
     3541 cttcagctga ctgcctatta tgtcgtgacc tggattgtgg gcgccaagcg ttaccatgat
     3601 ttggacattg attatattgg aatttggaat gagaggtcat ataatgccaa ttatattaag
     3661 atattaagaa aaatgctgaa ttatcaaggt ctccagcgag tgaaaatcat agcaagtgat
     3721 aatctctggg agtccatctc tgcatccatg ctccttgatg ccgaactctt caaggtggtt
     3781 gatgttatag gggctcatta tcctggaacc cattcagcaa aagatgcaaa gttgactggg
     3841 aagaagcttt ggtcttctga agactttagc actttaaata gtgacatggg tgcaggctgc
     3901 tggggtcgca ttttaaatca gaattatatc aatggctata tgacttccac aatcgcatgg
     3961 aatttagtgg ctagttacta tgaacagttg ccttatggga gatgcgggtt gatgacggcc
     4021 caggagccat ggagtgggca ctacgtggta gaatctcctg tctgggtatc agctcatacc
     4081 actcagttta ctcaacctgg ctggtattac ctgaagacag ttggccattt agagaaagga
     4141 ggaagctacg tagctctgac tgatggctta gggaacctca ccatcatcat tgaaaccatg
     4201 agtcataaac attctaagtg catacggcca tttcttcctt atttcaatgt gtcacaacaa
     4261 tttgccacct ttgttcttaa gggatctttt agtgaaatac cagagctaca ggtatggtat
     4321 accaaacttg gaaaaacatc cgaaagattt ctttttaagc agctggattc tctatggctc
     4381 cttgacagcg atggcagttt cacactgagc ctgcatgaag atgagctgtt cacactcacc
     4441 actctcacca ctggtcgcaa aggcagctac ccgcttcctc caaaatccca gcccttccca
     4501 agtacctata aggatgattt caatgttgat tacccatttt ttagtgaagc tccaaacttt
     4561 gctgatcaaa ctggtgtatt tgaatatttt acaaatattg aagaccctgg cgagcatcac
     4621 ttcacgctac gccaagttct caaccagaga cccattacgt gggctgccga tgcatccaac
     4681 acaatcagta ttataggaga ctacaactgg accaatctga ctacaaagtg tgatgtttac
     4741 atagagaccc ctgacacagg aggtgtgttc attgcaggaa gagtaaataa aggtggtatt
     4801 ttgattagaa gtgccagagg aattttcttc tggatttttg caaatggatc ttacagggtt
     4861 acaggtgatt tagctggatg gattatatat gctttaggac gtgttgaagt tacagcaaaa
     4921 aaatggtata cactcacgtt aactattaag ggtcatttcg cctctggcat gctgaatgac
     4981 aagtctctgt ggacagacat ccctgtgaat tttccaaaga atggctgggc tgcaattgga
     5041 actcactcct ttgaatttgc acagtttgac aactttcttg tggaagccac acgctaaCCA
     5101 GCTTTCTTGT ACAAAGTGGT TGATAGCTTG GATCCAATCA ACCTCTGGAT TACAAAATTT
     5161 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG
     5221 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT
     5281 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG
     5341 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC
     5401 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG
     5461 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT
     5521 TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC
     5581 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG
     5641 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA TCTGCCTCGA CTGTGCCTTC
     5701 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC
     5761 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG
     5821 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA
     5881 TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC GATTAGGATC TTCCTAGAGC
     5941 ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA CTACAAGGAA CCCCTAGTGA
     6001 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG
     6061 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA
     6121 ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC GCCAATTCGC CCTATAGTGA
     6181 GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG
     6241 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG
     6301 AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAG
     6361 CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT
     6421 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT
     6481 TAACAAAATA TTAACGCTTA CAATTTAG
//

